Determination of the sulfhydryl content of thymus and liver using DPPH
article
The sulfhydryl content of nuclei, mitochondria, microsomes, and supernatants from liver and thymus was determined spectrophotometrically at 522 mμ using diphenylpicrylphenylhydrazine (DPPH) as —SH reagent. The reaction is performed in an aqueous medium containing detergents and bile acids. Both the organic radical (DPPH) and the tissue fractions are solubilized in this system. By the use of this method, small amounts of sulfhydryl groups (0.01–0.2 μmole) can be detected directly without preceding extractions.
Topics
TNO Identifier
244437
Source
Archives of biochemistry and biophysics, 99(1), pp. 116-120.
Pages
116-120
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